Full Length Research Paper

Phylogenetic information reveals the peculiarity of Caryedon serratus (Coleoptera, Chrysomelidae, Bruchinae) feeding on

Cassia sieberiana DC (Caesalpinioideae)

M. Sembène¹*, K. Kébé¹, A. Delobel² and J. Y. Rasplus³

¹Département de Biologie Animale, Faculté des Sciences et Techniques, BP 5005 Dakar, Sénégal.

²247 Avenue Paul-Langevin, F-92260 Fontenay-aux-Roses, France.

³INRA - UMR 1062 CBGP (INRA/ IRD/ Cirad/ Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez, France.

*Corresponding author. E-mail: sembene@ird.sn or Mbacké.sembene@ucad.edu.sn. Tel: (00221)775541463.

Accepted 15 December, 2009

   Abstract

Studies over the past 20 years on natural populations indicated that sympatric speciation may be far more common and widespread among plants and animals than previously thought. By using molecular phylogenetics (on a combined data set of two genes), the relationships between Caryedon serratus native forms and forms feeding on groundnut was investigated. The specific objectives were to clarify the taxonomic status of C. serratus feeding on Cassia sieberiana DC. Morphological analysis was conducted, using parts of the adult specimens which were dry-mounted for the studies. Morphological study comprised extraction of the genitalia, which were cleared and mounted in Canada balsam following standard procedures. Results showed a strong differentiation of insects from different hosts’ trees, with specimens from C. sieberiana possibly representing a sibling species.

Key words: Caryedon serratus, phylogeny, taxonomy, Cytochrome B, ITS, groundnut, Cassia sieberiana.