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Application
of genefishing discovery system on differential gene
expression study for prokaryotic system
K. H. Chua1*, H. C. Chai1
and S. D. Puthucheary2
1Departments
of Molecular Medicine and University of Malaya, 50603 Kuala
Lumpur, Malaysia.
2
Medical Microbiology, Faculty of Medicine, University of
Malaya, 50603 Kuala Lumpur, Malaysia.
*Corresponding author.
E-mail:
khchua@um.edu.my.
Tel: 603-79676607.
Fax: 603-79676600.
Abbreviations:
DEG, GeneFishing Differentially Expressed Gene;
PCR, polymerase chain reaction; RT-PCR, reverse
transcription polymerase chain reaction; mRNA,
messenger RNA; rRNA, ribosomal RNA.
Accepted 3 June, 2010 |
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GeneFishing Discovery is a system applied on disease and
gene related research for eukaryotes and the starting
material for the study could be either total RNA or mRNA. In
this study we demonstrate the use of this discovery system
for a prokaryotic system by modifying the eukaryotic
protocol using the poly (A)-tailing approach. As an example
we compared the gene expression pattern of two closely
related species of bacteria under the same family (Burkholderia
thailandensis and Burkholderia cepacia) with
satisfactory results.
Key words:
Genefishing, differential expression, prokaryotic system. |
