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Antioxidant activity and phenolic compounds from
Colchicum luteum Baker (Liliaceae)
Bashir Ahmad
Centre of Biotechnology and Microbiology, University of
Peshawar, KPK, Pakistan. E-mail:
bashirdr2001@yahoo.com.
Abbreviations:
RSA, Free radical scavenging activity; 1H-NMR,
proton nuclear magnetic resonance spectroscopy; DPPH,
stable 1, 1-diphenyl-2-picrylhidrazyl; HR-EIM, human
resource-enterprise incentive management; HMBC,
heteronuclear multiple bond correlation.
Accepted 12 May, 2010 |
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The ethanolic extract from corms of the Colchicum luteum
Baker (Liliaceae) was investigated phytochemically.
During phytochemical studies, compounds 1 - 4 were isolated
from the n-butanol fraction. These compounds were
identified as colchicines 1, β - Lumicolchicine 2,
chlorogenic acid 3 and 3’, 4’, 5.7-Tetrahydroxyflavone 4, on
the basis of different modern spectroscopic techniques.
Chlorogenic acid 3’, 4’, 5.7-Tetrahydroxyflavone were
isolated for the first time from this species. On the basis
of scavenging activity of the stable 1,
1-diphenyl-2-picrylhidrazyl (DPPH) free radical, the crude
ethanolic extract and subsequent fractions of C. luteum
Baker offered promising antioxidant activity. The
highest activity was displayed by chloroform fraction (91%),
while the overall range was found (56 - 91%), expressed some
correlation with the isolated compounds.
Key words:
Colchicum luteum, Liliaceae, chlorogenic acid,
luteolin, antioxidant. |
