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Over-expression of ZmPti1, a homologue to Pti1,
increases salt tolerance of Arabidopsis thaliana
Huawen Zou1,2, Zhongyi Wu1,
Xiuhai Zhang1, Yongqin Wang1 and
Conglin Huang1*
1Beijing
Agro-Biotechnology Research Center, Beijing Academy of
Agriculture and Forestry Sciences, Beijing 100097, China.
2College
of Agriculture, Yangtze University, Jingzhou 434023, China.
*Corresponding author. E-mail:
conglinhuang@hotmail.com. Tel: 86-10-51503801. Fax:
86-10-51503980.
Abbreviations: SA, Salicylic acid; FW, fresh
weight; DW, dry weight; SOD, superoxide
dismutase; MDA, malondialdehyde; ROS, reactive
oxygen species; SOS, salt overly sensitive; MAPKs,
mitogen-activated protein kinases; MAPKK, mitogen-activated
protein kinases kinase; MAPKKK, mitogen-activated
protein kinases kinase kinase; HR, hypersensitive
response; PCR, polymerase chain reaction; WT,
wild type; Pti1, Pto-interacting 1.
Accepted 18 January, 2010 |
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Studies have shown that Pti1 plays an important role in
plant disease resistance pathway. However, Pti1 have not
been studied for its roles under salt stress condition.
Previous study has shown that maize ZmPti1 is induced
by salicylic acid (SA), low-temperature, mannitol and salt.
In order to analyze the further biological functions of
ZmPti1, ZmPti1 was over-expressed in
Arabidopsis. Under salt stress, compared to wild type,
transgenic plants grew better, had higher seedling fresh and
dry weight (FW, DW), seed yields and the superoxide
dismutase (SOD) activity; Seedling FW and DW of S1-1, S2-1
transgenic plants increased significantly than that of WT;
Seed weight of S1-1, S2-1 transgenic plants increased 71 and
106% respectively; However malondialdehyde (MDA) content and
relative electric conductivity (ion leakage) of transgenic
plants were kept to a relative lower level. Based on the
present knowledge, this is the first report that showed that
the over-expression of Pti1-like gene enhances the
salt resistance in plants.
Key words:
ZmPti1, transgenic Arabidopsis, salt resistance. |
