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African Journal of Biotechnology

     
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  Afr. J. Biotechnol.

  Vol. 9 No. 5

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  Kucuk S
 
  


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African Journal of Biotechnology Vol. 9 (5), pp. 755-759, 1 February 2010

ISSN 1684-5315  © 2010 Academic Journals  

 

 

Full Length Research Paper

 

Efficacy of tricaine on Peocilia latipinna at different temperatures and concentrations

 

Semra Kucuk

 

Department of Fisheries, Faculty of Agriculture, Adnan Menderes University, 09100, Aydin, Turkey. E-mail: skucuk@adu.edu.tr.

 

Accepted 11 December, 2009

 

   Abstract

 

The present experiment was designed to determine the effects of tricaine methanesulfonate as an anaesthetic for juvenile sailfin silver molly Poecilia latipinna at three different temperatures and anaesthetic concentrations. Silver molly (230 ± 25 mg and 24.07 ± 5.59 mm) were exposed to 150, 200, and 250 mg l-1 tricaine concentrations at 20, 25, and 30°C. Even though, a body of literature exist about the anaesthetic usage on foodfish species, not much information seems to be available on ornamental fish aquaculture. In the experiment, three tricaine methanesulfonate concentrations for each temperature were used to anaesthetize silver molly and recorded their induction, recovery times and survival. The necessary time to make anaesthesia on fish depended on concentration intensity and water temperature rise. When exposed to any of the concentrations, fish achieved a deep state of anaesthesia with induction times of 0.18 - 1.63 min and recovered fast in 1.53 - 2.08 min. To maximize safety and reduce fish mortality and stress, the minimum concentration producing desirable anesthetic effects was 150 mg l-1 at all three temperature.

 

Key words: Anesthetic, tricaine, Poecilia latipinna, temperature.

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