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In-vitro
plantlet propagation and microtuberization of meristem
culture in some wild and commercial potato cultivars as
affected by NaCl
Mansoor Saljooghian Pour*, Mansoor Omidi, Islam Majidi,
Dariush Davoodi and Parichehreh Ahmadian Tehrani
University of Tehran, Faculty of Agriculture (Karaj), Department
of Agronomy and Plant Breeding. P. O. Box 31578-11167, Iran.
*Corresponding author. E-mail:
Mansoor_781@yahoo.com.
Accepted
9 November, 2009 |
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The
effects of in vitro salinity (NaCl) on plantlet
growth, microtuberization and genetic diversity was
investigated under saline and non-saline conditions using
twelve cultivars (clones) of potato named Ranger-russet,
AGB-69-1, MEX-32,
Maine-28, Loman, Araucana INTA, Surena INTA,
American-INTA, Aracy, FLS-5, Agria and Marphona, which were
planted in the greenhouse. Young shoots were cut and
transferred to tissue culture laboratory of agricultural
biotechnology research institute. Apical and axillary
meristems were cultivated on MS medium and samples were
subcultured every four weeks on the same medium for plantlet
production. Then plantlets were propagated through single
nodal culture. To study the effects of salinity (NaCl) on
the growth of single nodal explants and on microtuberization,
cultivation on MS media with different concentrations of
NaCl (0, 25, 50, 75 and 100 mol l-1)
was carried out. Growth of single nodal explants on the
saline media indicated that all the characters of the
cultivars studied, the salinity levels and the studied
interaction effects had significant difference at P < 0.01.
These characters were also shown to have different responses
to salinity levels. Microtuberization responses on different
saline media was also different and indicated that all the
studied characters, except the number of microtubers and the
mean of minimum microtuber diameter, had significant
difference at P < 0.01 for cultivars, salinity levels and
interaction effects. Genetic diversity on the basis of
microtuberization using analysis of cluster among potato
cultivars and phenotypic correlations among characters under
saline and non-saline conditions were also studied.
Key words:
Potato, tissue culture,
microtuberization,
salinity tolerance, genetic diversity. |
