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The
relationship and combination effects on the promotion and
disease control of rhizospheric actinomycetes and entophytic
bacteria in tomato (Solanum lycopersicum)
Wang Mei-xia,
Qin Juan-juan, Chen Shuang-lin and Yan Shu-zhen*
Jiangsu Key Laboratory for Biodiversity and Biotechnology,
College of Life Sciences, Nanjing Normal University,
Nanjing 210046, China.
*Corresponding author. E-mail:
yanshuzhen@njnu.edu.cn.
Accepted 7 March, 2012 |
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Plants benefit extensively from endophytic and rhizospheric
microbes. They promote plant growth and confer enhanced
resistance to various pathogens. However, the interactions
of these beneficial microbes are not always well understood.
In this work, the interactions of two rhizospheric
actinomycete strains DK56 (Streptomyces microflavus),
ME2 (Micromonospora sp.) and endophytic bacterium
strains XG32 (Pseudomonas fluorescens biovar. IV) and DP1 (Bacillus subtilis)
were investigated via plate and broth cultivation. The
strains were tested singly and in combinations for promoting
the growth of tomato seedlings and biological control
against pepper blight disease. The results indicated that
there was nutrient competition between Micromonospora
sp. ME2 and the two endophytic bacterium strains while S.
microflavus DK56 competed with B. subtilis DP1
for nutrient when they were co-cultured in the plate. The
growth of endophytic bacteria was inhibited by actinomycetes
in broth. The plant growth promoting effect of endophytic
bacteria was not influenced by the presence of other
strains. Three strains S. microflavus DK56,
Micromonospora sp. ME2 and P. fluorescens biovar.
IV XG2 showed antagonistic activity
against Phytophthora capsici but they did not affect
each other on the control of blight disease of pepper when
co-inoculated with the sporangia suspension of P. capsici
and the control effect on pepper blight disease of
Micromonospora sp. ME2 could be enhanced by B.
subtilis DP1.
Key words:
Actinomycetes, endophytic bacteria, interaction,
growth-promotion, disease control. |
